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Cellafluor is a fluorimetric reagent that can be used for the assay of cellulase (endo-1,4-β-glucanase). The reagent consists of 2 components:
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1) 4,6-O-Benzylidene-4-methylumbelliferyl-β-cellotrioside
2) β-glucosidase (Thermotoga maritima)
Upon incubation with a cellulase (endo-1,4-β-glucanase) enzyme, the functionalised trisaccharide is cleaved and the ancillary β-glucosidase can act to hydrolyse the fragment containing the 4-methylumbelliferyl group. The rate of formation of 4-methylumbelliferyl is directly proportional to the cellulase (endo-1,4-β-glucanase) activity. The assay reaction is terminated and full fluorescence obtained from the fluorophore by the addition of Tris buffer.
Content: | 100 assays (manual) / 1000 assays (microplate) / 1000 assays (auto-analyser) |
Shipping Temperature: | Ambient |
Storage Temperature: |
Short term stability: 2-8oC, Long term stability: See individual component labels |
Stability: | > 2 years under recommended storage conditions |
Analyte: | endo-Cellulase |
Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
Detection Method: | Fluorescence |
Wavelength (nm): | 365 (ex), 450 (em) |
Reproducibility (%): | ~ 3% |